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TheSeed is an open annotation repository continuously allocating genes of known and often still unknown function to functional classes according to the available scientific knowledge [ 43].
Compared with our previous analysis (Kugler et al. 2013), the gene coexpression network here includes significantly more genes, as a result of the IWGSC bread wheat reference genome sequence, and allows allocating genes to their corresponding genomes.
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When gene multireads are significant, we have shown that our method gives better gene-level accuracy than the rescue method, which is used in Jiang and Wong (2009) for allocating gene multireads.
Gene ontology analysis allocated genes showing significant changes in inflammatory and, in a much lesser degree, to profibrotic pathways.
Since the distribution of genes among the six different temporal groups would be sensitive to the threshold E-value used to allocate genes.
The evidence rating system, described previously [ 17], allocates genes an overall score (ER1-5), indicating how compelling the bioinformatic and experimental evidence is for protein function.
Indeed, the allocated genes are still the same but are now localized on two contigs distant of 2 Mb from each other (NW_001494061 and NW_001494063).
Furthermore, when arm sorted chromosome datasets become available for a newly sequenced but not yet assembled genome, chromoWIZ can help to allocate genes to corresponding syntenic regions in barley, rice, bread wheat, and Brachypodium.
Overall, the algorithm has been able to allocate genes to pathways in a way that allows us to infer the state of individual pathways with increased certainty removing contradictions from the final results.
Only probes with allocated gene symbols and arithmetic mean intensity >50 for both channels were considered for further analysis.
However, in the expression arrays some genomic regions do not have enough allocated gene loci and the number of probes is sparse.
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