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We utilized both multi-partite fusion constructs in vaccination experiments in order to test whether the utilization of M2 in a fashion that alleviates its cytotoxicity is capable of providing additional protective benefit (Fig. 4).
SIRT1 deacetylates p53 at its C-terminal Lys382 residue and alleviates its transactivation activity.
Elimination of these corepressors by proteolysis exposes Rgt1 to phosphorylation and alleviates its repressive activity (Palomino et al. 2006).
In mammalian cells, mutation of two lysines in HDAC1 decreases its sumoylation and alleviates its repressive activity (David et al. 2002).
Binding of Hh to Ptc, which is facilitated by the Ihog/Cdo family of coreceptors, alleviates its inhibition of Smo, leading to Smo phosphorylation and activation.
The KIN-2 regulatory subunit functions by binding and inactivating the catalytic subunit in the absence of cAMP; binding of cAMP to KIN-2 alleviates its inhibition of KIN-1 activity (reviewed by Taylor et al. 1990).
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