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However, RNA silencing may not be the only factor limiting amplicon expression in many occasions, for instance it has been reported that a mutant version of Cucumber mosaic virus (CMV) lacking its silencing suppressor 2b was nevertheless able to alleviate the repression of a Potato leafroll virus (PLRV) amplicon [ 17].
Luciferase reporter assays confirmed that hairy1 TP is able to partially alleviate the repression of miR-9 on the hairy1 luciferase reporter when introduced in vitro together with miR-9 mimics, but not of a reporter carrying the 3′UTR of other miR-9 targets such as hairy2, TLX, and Onecut1 (Plaisance et al., 2006).
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In the presence of low levels of glucose, Snf1p phosphorylates and releases the DNA bound Mig1p, which is subsequently exported from the nucleus, alleviating the repression of glucose-repressed genes [ 16].
In addition, overexpression of this sirtuin alleviated the repression of HR repair observed during replicative senescence.
The discovery of this apparent host-pathogen signal communication pathway and the finding that exogenous spermidine alleviates the repression of calcium on T3SS gene expression (Figure 4A, 4B) seem to provide a useful platform to resolve this puzzle.
Knockdown of RISC core component GW182 alleviated the repression of BRCA1 reporters, regardless of the presence of the downstream introns.
In the presence of both miRNAs, the wild-type chimeric reporter was repressed efficiently, while mutating either the ORF miRE or the 3' UTR miRE alleviated the repression.
Given that SroC alleviates the repression of these multiple ABC transporters, we expected this sponge to promote growth under peptide-limiting conditions.
In the presence of both miRNAs, the wild-type chimeric reporter was repressed most efficiently, while mutating either the ORF miREs or the 3′ UTR miRE alleviated the repression.
The native crp gene was replaced by a cAMP-independent mutant to alleviate the catabolite repression in the presence of glucose.
Sumoylation (addition of ubiquitin-like modifier SUMO to the lysine residues) of ZEB protein at Lys391 and Lys866 by the polycomb protein Pc2 could alleviate the E-cadherin repression mediated by ZEB proteins [ 33].
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