The phrase "allelic replacement" is correct and usable in written English.
It is typically used in genetics and molecular biology to refer to a technique where one allele of a gene is replaced with another allele.
Example: "The researchers employed allelic replacement to study the effects of the mutated gene on the organism's phenotype."
Alternatives: "gene replacement" or "allele substitution".
E. coli strains containing different gltA, iclR, and arcB alleles were constructed using the pKO3 allelic replacement method (Link et al., 1997).
The mtaR mutant bears a kanamycin cassette insertion in the 5' region of the mtaR derived by precise allelic replacement of the wild-type mtaR allele [ 11].
Song, J. H. et al. Identification of essential genes in Streptococcus pneumoniae by allelic replacement mutagenesis.
Allelic replacement of PorB3 with PorB2, in both encapsulated and unencapsulated strains, confirmed the role of PorB2 in AP inhibition.
Arnaud, M., Chastanet, A. & Débarbouillé, M. New vector for efficient allelic replacement in naturally nontransformable, low-GC-content, gram-positive bacteria.
"A Modified Janus Cassette (Sweet Janus) to Improve Allelic Replacement Efficiency by High-Stringency Negative Selection in Streptococcus pneumoniae". PLoS ONE 9 (6): e100510.
Thus, SJ enhances negative selection stringency and can accelerate allelic replacement in pneumococcus, especially when transformation frequency is low due to strain background or suboptimal transformation conditions.
The Janus cassette permits marker-free allelic replacement or knockout in streptomycin-resistant Streptococcus pneumoniae (pneumococcus) through sequential positive and negative selection.
For counterselection, we adapted a strategy using I-SceI, which causes a double-stranded break in the integrated suicide vector, forcing a second recombination to mediate allelic replacement.
To estimate the fitness effect of the synonymous mutations themselves, we used an allelic replacement strategy to introduce each synonymous mutation into the ancestral genetic background that lacked the Δ(PFLU4435 4466) mutation (see Methods).
Fitness of the SBW25 allelic replacement constructs was estimated relative to SBW25-lacZ in glucose, succinate and mannitol environments to determine whether the detected fitness improvements were glucose specific.
