Exact(14)
cdc22 levels were normalized to adh1 and then all time courses were normalized to asynchronous wild-type controls included on all gels.
Two milliliters culture medium per well was sufficient to sustain cell growth over all time courses investigated.
Note that all time courses display high expression at early time points but distinct expression patterns at later time points.
All time courses of red/green ratios for all ESTs were sorted via self-organizing maps with Euclidian distance [ 21, 22] (25 cluster (5 × 5) neuron map).
All time courses were then realigned to the 3D7 transcriptome and to each other when performing pairwise comparisons (Additional file 3: Figure S2).
Also as all time courses showed an increase in fluorescence over time, quenching did not appear to be a significant contributor to the results.
Similar(46)
For recycling experiments, after 30 min uptake and addition of TCEP, a series of sequential images was taken at 1 min intervals for 10 min. All time course measurements were with identical microscope settings and below saturation levels.
Mock- infected flight RNA levels were normalized against ground control to obtain the pre-infection level, and all time course points thereafter, for ground and flight, were normalized against their respective pre-infection level.
All time course and endpoint experiments were performed a minimum of 5 times each.
All time course experiments for Southern analysis were repeated at least twice with similar results.
All time course expression profiles, including COSOPT and JTK_CYCLE outputs, can be viewed on our publically accessible database, Bioclock[ 58].
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