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Specific potential lung toxicity of organic extracts of PM0.5 will be assessed in vitro on a total of 10 unique samples, obtained by mixing the organic extracts from all the samples individually collected from each town and each season.
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In all experiments, all the samples were assessed individually.
Duplicate reads were removed and single nucleotide polymorphisms (SNP) and insertion/deletion (INDEL) discovery and genotyping was performed on all samples individually using the GATK HaplotypeCaller producing a standard variant call format (VCF) [ 20].
In all cases, the samples were individually coded so that the different data pertaining to the same animals could be compared.
All samples individually sealed in water impermeable bags were thawed in water at 10 °C and then analysed.
We handled all samples individually and immediately after retrieval from storage.
While desirable, analysis of all samples individually would have been prohibitively expensive.
We individually genotyped the LCE3C_LCE3B-del for all the samples available from the 13 populations used in the aCGH analysis.
Each level of abstraction provides the user with more power; they can choose to deal with wells individually, all the wells of a plate, all the samples from one subject, all the plates containing a sample from one subject, etc.
The data on different distribution of DDTs in the wet and dry season in water and sediment were showed in the tables 3 and 4. To compare these results with over all period of sampling times, Figure 7 provides the error bar over all samples for water and sediment samples individually based on DDTs metabolites and HCH isomers.
These initial values are sampled from a Gaussian distribution with a mean value that may be specified by the user for each sample, or for all samples without individually specified values by a command line parameter.
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