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The raw reads expression profile generated for all the replicates of the samples were subjected to Trimmed Mean of M-values (TMM) normalisation using the bioconductor package edgeR [ 30].
Among all samples, all the replicates belonging to respective samples clustered together.
Most of the SES were same for all the replicates but in case of variation mode was taken.
The area was standardized in number of pixels for all the replicates, and the ImageJ software assigned a value for the selection based on the intensity of each pixel.
Case 3: L i n i B D ≤ T < L B D. In this case, <span class="lh">all the replicates of the target data item in the m-th broadcast cycle were broadcast when the mobile device began to wait.
Into half of all the replicates the parasite O. bayeri was added, to measure fitness effects in the presence of parasites, while the other half of the replicates stayed free of parasites.
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Referred to as the "Av Repl-Fo CV", averaging the quantitative variances generated by all the replicate reaction sets within a run, provided such a general assessment.
The other 9 compounds gave no positive chemotactic events in all the replicate experiments.
For all three experiments, after normalization, the average and standard deviation (SD) of the log2 transformed ratio for all the replicate spots for each gene was calculated.
PCA was performed on all the replicate RBC samples from individual stages of the parasite life cycle following spectral data preprocessing.
All of the replicates were highly reproducible, supporting the purity and reliability of the method.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com