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For all the protocols described above, untreated controls were processed identically, the only difference being the deletion of the stimulant/inducer from the incubation.
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In all of the protocols described, data from each experiment were obtained from a different animal or patient.
All other steps followed the protocols described above.
All protein expression and purification followed the protocols described in our previous study (Zheng et al., 2014).
For all groups, the protocol described a number of subsequent treatment steps for patients whose response to therapy was insufficient, based on the disease activity score (DAS) in 44 joints of more than 2.4.
All mice underwent the protocol described in Fig. 1.
MLST with seven housekeeping genes [ 20], adk (adenylate kinase), fumC (fumarate hydratase), gyrB (DNA gyrase), icd (isocitrate dehydrogenase), mdh (malate dehydrogenase), purA (adenylosuccinate synthetase), and recA (ATP/GTP motif), was performed on all isolates according to the protocol described on the E. coli MLST website (http://mlst.ucc.ie/mlst/dbs/Ecoli/documents/primersColi_html).ucc.ie/mlst/dbs/Ecoli/documents/primersColi_html
Macrorestriction of the genome and PFGE were conducted on all MRSA isolates according to the protocol described by Murchan et al. (15 ) and included on each gel with EMRSA-15 and -16 and CMRSA-5.
All samples were digested according to the protocol described above (see section in-solution digest).
The protocol described here meets all of these criteria.
The assay was performed according to the protocol described previously46,47.
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