All transcription templates were verified by sequencing.
Each qPCR product was verified by dissociation curve analysis, and all the qPCR products of two cDNA templates were verified by 2.2%% agarose gel electrophoresis that showed single band with correct amplicon length and no primer dimmer.
The system can be used with any biometric modality where a feature extraction method yields a fixed size binary template and a query template is verified when its Hamming distance to the database template is less than a threshold.
The absence of DNA template was verified by replacing the RT/Taq mix by the Taq DNA polymerase only.
Presence of amplifiable template was verified using mitochondrial DNA primers C1-J-2630 and TL2-N-3014 [ 55] and universal invertebrate ribosomal primers BD1 and 4S [ 56, 57]; if product was lacking the sample was discarded.
All the plasmids were verified by sequencing.
All the clones were verified by sequencing.
All the sequences were verified by sequencing.
All template-generated event identities were verified by visual inspection of the record.
All inserts were verified by sequencing.
All constructs were verified by Sanger sequencing.
