The identities of test and stimulus mice within a social group were determined randomly on the first day of SI testing and remained constant for all subsequent tests.
The control Vs virus and pGGVs virus, recovered from the infectious clone (see Methods), produced high positive HA titres (1∶640-1∶1280) at pH 6.2 and this was therefore the pH of choice for all subsequent tests (Table 1).
The sediment in each tube was reconstituted with phosphate buffered saline (PBS) to 2.5 ml, mixed well and then pooled into one tube that served as common inoculum source for all subsequent tests.
At weaning, mice were formed into a social group (2 mice from each sex) that served as the general housing arrangement for all subsequent tests, with the exception of Experiment 4 (see below).
All subsequent testing was conducted in these cages.
The ergometer saddle and handle bar height configuration was recorded and reproduced in all subsequent tests.
However, performing all subsequent tests separately for right and left frontalis muscles did not give deviant results from those reported.
Group housed C57Bl/6J 9 weeks old male mice underwent stereotactic surgery, after which they were singly-housed throughout all subsequent testing.
Of these 553 patients, the majority (87%; 479 of 553) remained negative at all subsequent testing over 18 months (Table 3).
A strain of 1% was below the LVE limit for each hydrogel and was used for all subsequent testing.
