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For the in-gel kinase assays shown in Figure 3A,B and Figure 3 figure supplement 2 all steps except the equilibration in reaction buffer and the reactions were carried out together and exactly the same way which allows the autoradiographs to be compared.
EUTelescope provides processors for all steps, except for the conversion of the DUT raw data, marked with a dashed outline.
All steps, except antibody incubations, were carried out in net inserts (Corning incorporated) placed in wells of 24 well plates.
All steps except the "Final map construction" were identical to the procedures described above, since [26] used JOINMAP for that part of the analysis.
All steps except the blocking step were carried out in 100 µl volumes and at room temperature except for ELISAs involving dengue virus which were done at 50 µl volumes.
All steps, except where mentioned, were performed at 4°C.
Similar(48)
Negative controls were processed following all the steps, except for exposure to anti-AQP1 antibody.
Follow all the steps except putting in the roller.
The remaining 27 haplotypes share a common origin, with most differing by just single mutational steps, excepting haplotype 20 from the North River (site Y), which differs by eight.
All purification steps, except for gel filtrations, were carried out at 4°C.
We performed all major steps, except the extraction of the quantitative data in duplicate.
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