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The macroscopic reaction rate constant is set to k = 3.78 × 106 l/(mol·s), which is fairly fast but not extremely diffusion limited (k D = 3.78 × 107 l/(mol·s) for D = 1 μm/s, and k D = 3.78 × 108 l/(mol·s) for D = 10 μm/s) - see Additional file 1, Section 3. Since all setups are conducted with the same number of enzymes and the same reaction rates, they produce similar results (see Figure 6c).

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All setups were run in duplicate and inoculated with the ASC of one of the three WWTP (small-scale setups) and with ASC from CAS-M (larger-scale setups) to 4 and 2 g/L mixed liquor suspended solids (MLSS), respectively.

All setups were done in duplicate.

All the setups were tested from 25 °C up to 500 °C.

To test that the setups are working, all the packets are received by the base station, which indicates packet reception through a terminal printout.

All model setups were initialized according to the inventory data for Wetzstein and Tharandt (Table 1).

Additionally, all test setups were compared regarding four aspects: method for angle calculation, system for data acquisition, loading condition and testing rig design.

At the end of the experiment, where all four setups were analyzed by FISH, the Betaproteobacteria dominated the activated sludge closely followed by the Alphaproteobacteria.

While the interconnect prolongs the critical path for memory accesses, all investigated setups were still able to run at clock frequencies of up to 800 MHz.

All PCR setups were done according to the manufacturers protocols.

All PCR setups were performed at least in triplicate.

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