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As a secondary model for analysis, the final 20 items were also subjected to a traditional item analysis.

Cluster is a random effect, in a secondary model the interaction cluster*time is also included as a further random effect.

For a suitable prediction of ricin inactivation, a secondary model for every interim product in the food chain would be necessary.

The primary PLS models contained all individual x and y variables, while the secondary models contained all or defined blocks or sets of influential (VIP > 1.0) x variables and excluded the occlusal caries y variables in the Y matrices due to individual Q-values below 0.1 (10%).

We then investigated secondary models formed from all influential biological (n = 18) or previous caries (n = 17) multimarkers alone or combined for ability to predict 2-year caries increment (Table 1, Fig. 2B).

We next compared secondary models formed from all influential biomarkers (n = 18 multimarkers), a five biomarker panel and a single lactobacilli marker, for ability to predict baseline caries (Table 1, Fig. 2A).

Two secondary models were also produced.

(D ) Classification of primary and secondary models (left).

As a consequence, the estimated Arrhenius-type secondary models were used to create a combined primary/secondary model which was saved into the PMM-Lab model database.

The secondary models harbor influential (VIP > 1.0) markers from the corresponding primary models M1 to M4.

VIP-values are also used for variable selection for the generation of secondary models.

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