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All samples were viewed in a JEOL JSM-6360LV SEM (JEOL, Tokyo, Japan) operating at 25 kV at 5000× level of magnification.
All samples were viewed under UV radiation using a Zeiss Axiovert inverted microscope (filter set 09, 450 490-nm excitation and 515-nm emission, Carl Zeiss, Jena, Germany) and the number of fluorescing cells recorded as a proportion of the total number of cells (two samples taken with a minimum of four fields of view counted).
All samples were viewed with a ZEISS FEG scanning electron microscope at 1 kV.
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For comparison of birefringence intensity in POL images, all fibre samples were viewed at the same angle relative to the optical axis - the angle that maximized the intensity of birefringence in secondary wall stage fibres.
Samples were viewed under the Olympus IX81 microscope.
Mounted samples were viewed with an Hitachi S-4800 (Ibaraki, Japan) scanning electron microscope at 10 kV.
Samples were viewed using a Zeiss LSM 510 META confocal microscope with a Plan-Apochromat 163 and 100×/1.4 Oil DIC objective.
Samples were viewed at 80 kV on a Hitachi 7600. (A) Images were captured at a magnification of 30,000× to gain an overview of cellular changes.
Samples were viewed on a Keyence BZ-X700 microscope (Keyence, Osaka, Japan), following mounting with ProLong Diamond Antifade Mountant with 4,6-diamidino-2-phenylindole (DAPI Thermo Fisher Scientificc).
Samples were viewed under microscope a Nikon Eclipse 90i and a confocal D-Eclipse C1.
Samples were viewed by light microscopy to determine the presence of multilocular droplets.
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