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All samples were then incubated in 0.5% PBT with 5% NGS, 0.1% sodium azide containing the secondary antibodies for 24 h at 4 °C.

All samples were then amplified by PCR using NEBNextHigh-Fidelity 2× PCR Master Mix (New Englands Labs) with primers containing a barcode to generate libraries.

All samples were then extracted by phenol/chloroform and the free DNA contained in the upper phase was quantified by RT-qPCR.

All samples were then rinsed several times with ethanol and dried under argon atmosphere prior to use.

All samples were then stored in distilled water for 24 h at 37°C, except the long-term specimens of the first phase of the study, which were stored for 6 months (Figure 1).

All samples were then dialyzed against 8M urea.

All samples were then sequenced to confirm the accuracy of the genotype classification by RFLP.

All samples were then analyzed with a FACScan flow cytometry as described above.

All samples were then stained with Streptavidin-PECy5 (BD Bioscience) and acquired by flow cytometry.

All samples were then diluted to 1 mL with sample diluent (HBS-EP that contains 0.1% tween 20).

All samples were then incubated at 37°C for an additional 24h before processing with crystal violet as described above.