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The expression values for a gene across all samples were standardized to have mean of 0 and standard deviation of 1 by linear transformation.
All samples were standardized by random subsampling to 1885 reads per sample (based on the smallest sample size by default), using the sub.sample command (http://www.mothur.org/wiki/Sub.sample), for calculating richness (OTUs at ≥99% sequence similarity), rarefaction curves, diversity and community compositional indices and matrices to be used later for the statistical analyses.
Sequences in all samples were standardized to 23,554 for further analysis.
Each reaction was performed in duplicate, and all samples were standardized using the internal control glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene and 18S rRNA.
All samples were standardized to the same protein concentration for the following assays.
All samples were standardized to equal RNA concentration using the RiboGreen RNA quantification kit (Molecular Probes, Leiden, Netherlands) and measured in duplicates.
Similar(50)
All the samples were standardized to 500 ng/μL, and equal volumes of the tissue samples from different individuals in the same group were combined into one pool.
Then, all the samples were standardized to zero mean and unit variance.
In all datasets, samples were standardized to zero mean and unit variances before other analyses were performed.
Handling and processing of samples were standardized for all patients.
It should be noted that although the RNA samples were standardized across all replicates, the Gene Array and Exon Array experiments were processed in different labs, and under slightly different protocols.
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