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Since all samples were selected for the DNA extraction based on the histological observation of high tumor cell content, the identification of primary cases without or with low methylation can not be due to a reduced number of tumor cells.
All samples were selected randomly, and different farms were chosen in different seasons.
For the comparison of Figure 3B, PC species that were detected in all samples were selected.
All samples were selected from GC cases diagnosed in our department from 1997 to 2011.
All samples were selected and reviewed by an experienced pathologist and co-author (L.
All samples were selected from the same series of cancers as we used in a previous study [ 10].
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Only T-ALL samples were selected for NOTCH1 and RELA in order to match cell population used for creation of the respective gold standard.
The libraries derived from a CD34+ HSC sample, a Jurkat leukemic T-cell line, and all libraries generated from normal T-cells or T-ALL samples were selected for the comparison.
For genes with multiple probe sets, the probe set showing the strongest signal intensity across all samples was selected and all others were excluded.
The molecular features (genes for expression data and probes for DNA methylation data) with largest variances across all samples are selected to construct datasets of different sizes.
All the study samples were selected within the same age group, as different periodontal issues such as gingival recession tends to be affected with age [15].
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