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Next, all samples were sectioned in two perpendicular directions to the bonded interface using a water-cooled diamond saw at low speed (Isomet 1000, Buheler Ltd ,Lake Bluff, IL, USA) to obtain beam-shaped specimens with approximately 1.0 (±0.1) mm2 of transverse-sectional area [17].
All samples were sectioned at 7 µm.
All samples were sectioned at random angles resulting in cross and longitudinal sections of mitochondria.
To analyze morphological changes, all samples were sectioned and reoriented in successive slices to identify those containing the longest villi.
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The samples were sectioned at 1 um thickness for light microscopy and thin-sectioned for electron microscopy (70 nm thick).
Frozen samples were sectioned at 30 µm for staining, paraffin samples were sectioned at 20 µm.
Paraffin embedded skin samples were sectioned at 4 µm.
The frozen samples were sectioned and stained with Oil Red O and Sudan III.
After embedding in Killik and freezing in dry ice, samples were sectioned in the coronal plane.
Samples were sectioned at 14 µm and then mounted as above.
Formalin-fixed samples were sectioned at 4 µm and stained with hematoxylin and eosin.
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