Sentence examples for all samples were required from inspiring English sources

All samples were required to be estrogen and/or progesterone receptor positive by ligand-binding assay and had received tamoxifen monotherapy only in the adjuvant setting.

For quality control, all samples were required to have successful genotyping using Illumina's BeadStudio v. 3.3 software for at least 98% of the SNPs.

All samples were required to be estrogen (ER) and/or progesterone receptor (PR) positive by ligand-binding assay and had been prescribed tamoxifen monotherapy for 5 years post diagnosis as adjuvant therapy.

To achieve this goal, experimental work, including measurement of equilibrium adsorption isotherms and XRD characterization of all samples, is required.

In brief, all blood samples were required to be transported within 12 hours of collection, all plasma samples were required to be extracted and stored at −20°C within 12 hours of arrival, and all tests were required to be conducted at laboratories certified by the National Center for AIDS/STD Control and Prevention (NCAIDS).

All no-target samples were required to give a failing test result.

All the other breast tissue samples were required in their entirety for the ELISA and immunohistochemistry experiments.

Plasma samples were required to have HIV-1 RNA levels ≥5,000 copies/mL at all time points analyzed.

At least three tumour samples were required for each patient.

According to the calculated formula for sample size, a minimum of 983 samples were required.

A minimum sample size of 43 colostral, 39 serum and 54 milk samples were required.