Unit cell constants of the samples were refined with the PDXL software program provided by Rigaku Corporation.
Variants in the unpaired cell line genomic data were identified using SAMtools [ 15]; the indel lists for these samples were refined to include only those events that were also called through de novo assembly.
An approach to overcome this is to iteratively compare experimental images with simulations [5, 6]; imaging parameters and model structure of the sample are refined until the simulated and experimental image match.
Thirdly, in rural areas, sampling was refined to the village level.
For this study, the Q-sample was refined from an earlier concourse, a collection of statements comprised of experiential, theoretical and evidence-informed viewpoints, collated by two of the authors of this study.
At the last cycle, all the parameters were refined simultaneously.
All structures were refined using REFMAC5 (Murshudov et al., 1997).
Finally, anisotropic displacement parameters for all sites were refined.
The reads obtained from all samples were assembled using Trinity [ 23] and refined using Cdhit-EST [ 24] to produce a transcriptome of ~170 000 contigs with an average read length of 745 bp.
All B-factors were refined as isotropic with TLS parameterization.
