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All samples were calibrated according to the expression of the EF1α gene that was analysed using primers 1818 and 1819.
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Samples were calibrated with Peptide Calibration Standard (Bruker).
Calibration coefficients in the synthetic samples were calibrated independently using standards prepared at the minimum, median, and maximum concentration of each metabolite in the synthetic mixtures (Table S-1 in Supporting Information).
The samples were calibrated in a thermocouple calibrating furnace in temperature range from 200 °C to 950 °C.
The binding energies for the samples were calibrated by setting the measured binding energy of C 1s to 284.60 eV.
In both techniques, samples were calibrated using data collected in a well-characterized methane/air diffusion flame supported on a Wolfhard-Parker slot burner.
Samples were calibrated with a 2-MQ internal standard.
Samples were calibrated against DNA from male-nontreated control mouse.
Amplification results of affected samples were calibrated against those of controls to obtain the differences in gene expression.
Samples were calibrated against a calcium standard (10mg/dl, Calcium kit, Sigma and Procedure No. 0150, Stanbio Laboratory), and absorption was measured at 575 ± 5 nm using a spectrophotometer or microplate reader.
The mobile phase was THF with 5% triethylamine (TEA) eluent at a flow of 1.0 mL/min, and samples were calibrated against Varian Polymer Laboratories EasiVials linear poly styrene) and poly methyl methacrylate) standards (162 2.4 × 10 g/mol) using Cirrus v3.3.
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