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As the process to reduce the carbonate content can influence δ15N values freeze dried whole-body, tail-muscle, and zoo- and phytoplankton samples were allocated into two groups.
Nevertheless, samples were allocated to biclusters with consistency in both molecular and clinical characteristics.
Samples were allocated randomly for individual and survey into 96-well formats.
The focus was examination of Ca2+ influx but samples were allocated to the other assays if cell number was sufficient.
Samples were allocated randomly to 96-well plates for bisulfite conversion, amplification and pyrosequencing with technical replicates in each plate.
For the statistical analysis, samples were allocated into two groups: normal lactate levels (Group 1) and hyperlactatemia (Group 2).
The quadriceps and biceps samples were allocated by a 11×11 Latin square design for each group, as described previously.
A household would be the basic sampling unit in each kebele and samples were allocated proportionally to each based on their total household.
The samples were stratified by map category - 431 samples were allocated to the category 2 and 3, and 87 to category 1.
Serum samples were allocated to 96-well plates so that age, gender, study site (UK, Japan) and disease group (control, gastric cancer) were evenly distributed.
A total of 16 injured samples were allocated to histological and immunohistochemical analysis (comprising four in each group) and another 16 allocated to western blotting test (four in each group).
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