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For example, the expression level of GW112 was below the sensitivity of this quantitation system in all samples and, therefore, this gene was not included in subsequent analyses.

This approach effectively excluded probes that displayed constant intensity in all samples and therefore did not contribute to distinguishing between the two groups.

Of 59,479 total contigs, 28,053 had a mean of greater than five counts and a standard deviation less than the mean across all samples and therefore were used for gene expression analysis.

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A >threefold difference cutoff was >sixfold the standard deviation between replicates of all samples and was therefore highly significant.

The levels of the Aβ isoforms Aβ1-13 and Aβ1-20 were low, were not detected in all samples, and were therefore omitted from further analysis.

These genes display consistent ratios of expression across all samples, and were therefore used to calculate size factors to normalize the VR expression estimates (which had previously been normalized for depth of sequencing).

The concentration of polycarbonate in DMF and the physical parameters of the deposition process, such as the spinning speed and duration, are the same for all the prepared samples, and therefore, the observed morphology is mainly the result of the properties of the monomers.

Genes that showed expression levels of 100 across all 24 samples, and therefore lacked variability, were then removed from the analysis.

Finally, we do not have any unassigned peaks and the proportion of anion signal is correct for all our samples, and therefore, the amount of acetylation of our carbohydrates, if any, must be small <5% (w/w).

If this does occur, then it is likely to cause some underestimation, but this should happen in all samples and is not, therefore, expected to cause any systematic bias.

The particular advantage of neutron diffraction is that neutrons penetrate deep inside a sample and therefore probes structural changes inside the bulk of a material.

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