Sentence examples for all samples and subsequently from inspiring English sources

To test for gene expression correlations, read mapping counts obtained with Novoalign for all 63,683 reference transcripts were filtered removing in a first step transcripts expressed in less than 10% of all samples and subsequently with less than 1 million reads in fewer than two samples.

Optimism is finally averaged across all bootstrap samples and subsequently subtracted to the original classification indexes computed with the actual dataset.

Cell aliquots were harvested at each data point in Figure 2. Cells were diluted to an optical density at 600 nm = 0.1 for all samples and then were subsequently fixed by the addition of 0.25% formaldehyde (final concentration).

After above-mentioned general experimental procedures were followed, DCF-DA (200 μl; 10 μM) was pipetted to all the samples and were subsequently incubated for 60 min in a humidified atmosphere (37 °C, 5%% CO2. Fluorescence was measured at excitation wavelength of 485 nm and emission wavelength of 520 nm using fluorometrics (Department of Pharmacology, University of Pretoria).

All samples were subsequently aliquoted and frozen prior to analysis of protein and autoantibody biomarkers.

All samples were subsequently washed and dehydrated through a graded ethanol series and embedded in epoxy resin.

These were kept separate from the train sample, and subsequently processed through the image classification.

Experimentally the excitation volume was first focused above the surface of the sample and subsequently moved into the sample in step sizes of 100 nm, while rastering laterally.

At least 5.000 events were acquired from each sample and subsequently analyzed with Lysis II software.

All samples used subsequently had RIN values between 9 and 10 [ 20].

After sintering, all samples were subsequently heat treated up to 192 h at 1450 and 1300 °C.