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The islands are all protrusions of parallel submarine ridges trending from the northwest to the southeast.
To assess the topographic effects (i.e. with or without nanostructures), the prepared polyaniline nanoprotrusion structure surface was rubbed at saturation level with a rubberised cloth to flatten all protrusions [11].
For disappearances, we counted all protrusions (spines and filopodia) that could no longer be identified on the next observation.
The measures of density, turnover and stability were carried out by analyzing all protrusions, i.e. filopodia and spines.
The time and distance of all protrusions that were parallel to the line of analysis during the 15-minute period were used to calculate the mean protrusion velocity.
All protrusions were measured on all dendrites within the field of view and an observer blinded to the transfection type did all analyses.
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First, the smallest number of pseudopods that cover no less than 50% of all protrusion and retraction activities is selected.
All other protrusions, consisting mostly of thin and stubby protrusions, are scored as "other".
Biocytin injections confirmed that all dendritic protrusions were labeled with EGFP-F [31].
We should note that since we defined all thin protrusions as filopodia, some filopodia longer than 10 µm might be defined also as branches in the analysis shown in Figure 5B.
In the treated animals, we found that of all quantified protrusions (∼90%) appeared to be spines.
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