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Written informed consent was obtained for all procedures, with participants being free to withdraw at any time without penalty.
The MAIR-IR spectroscopic instrument was adjusted during all procedures with the IR spectra wave number ranging from 4,000 to 600 cm-1, transmission in percentage, 10× scan, and 4-cm-1 resolution.
All procedures with siRNA treated cells were followed as previously described [18].
All procedures with animals were performed after general anesthesia with ketamine chlorhydrate (Rhone-Merieux, Lyon, France).
All procedures with animals were carried out in accordance with institutionally approved protocols.
All procedures with animal subjects have been approved by the Ethics Committee for Use of Animals – CEUA from Universidade Federal do Rio Grande do Sul (protocol number 2008058).
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"I went through all the procedures with the referees.
All the procedures with animals were carried out in accordance with the approved protocols of Indian Institute of Science.
All the procedures with animals were carried out in accordance with approved protocols of Indian Institute of Science, Bangalore.
All the procedures with animals were carried out as approved by the animal ethic's committee of the Institute.
All the procedures with animals avoided or minimized discomfort, distress, and pain to the animals.
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