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Furthermore, to decrease the staining bias, we used automated machine for all procedure of IHC staining.
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All procedures of housing and treatment of animals were performed in accordance with IACUC regulations.
All procedures of cDNA synthesis and RT-PCR analysis were performed as described [22].
All procedures of the animal study were approved by the Ethics Committee of National Research Institute for Family Planning, China (Approval ID: 20100318).
Before participating in the experiment, all recruited students and their teachers were informed about all procedures of the experiment and written informed consents were obtained.
All procedures of the study will be confidential.
Design and all procedures of the present study were in accordance with the Declaration of Helsinki.
All procedures of animal experiment of this study were approved by the Life Science Ethics Committee of Zhengzhou University.
All procedures of epithelial removal were performed on the right eyes of experimental animals.
All procedures of the study will be carried in compliance with the Helsinki Declaration.
JWK performed all procedures of the statistical analysis, participated in the discussion and edited the manuscript.
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