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According to the neutrality theory, a large proportion of all possible mutants at any gene locus are harmful to their carriers.
Observe that this represents only 8.42%% (11,250/133,683) of all possible mutants μJava is able to generate considering the entire mutation operator set.
The average λ for different sequence length was obtained through comparing the secondary structure of randomly created RNA sequences with that of all possible mutants with only one substitution.
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All possible mutant combinations were recovered by plasmid shuffle, indicating that mutation of either or both sites does not affect cell viability.
The part of S gene we chose for sequencing has many drug-resistant mutant sites, and we detected all possible mutant sites in this region of 145 samples and found the rtM204V mutation in one sample.
In designing a screen of this type, there are trade offs between the advantages of mutating multiple codons and the disadvantages of needing to screen large numbers of transformants to recover all possible mutant combinations.
This means that all the possible mutants were generated.
This sequence had 19 possible mutations and using a Sun Fire X4100 Linux workstation (AMD Opteron Processor, 2.2 GHz, 4.0 Gigs RAM), this algorithm needed 3 h and 40 min to calculate the LSE scores for all the possible mutants.
Given the observed mutational load of 1.8 errors per sequence (based on sequencing 48 nonselected transformants) and the average library size, one expects that the transformation mixture contains at least one copy of all 2583 one-step mutants, 28% of all possible double mutants, and only 0.04% of all possible triple mutants (http://guinevere.otago.ac.nz/cgi-bin/aef/pedel.pl).pl
This line was maintained as a possible mutant line with an altered sleep-wake phenotype because it showed increased wake (820±21 min) over 24 hours compared to wild-type B6 males (718±49 min), and hence the line was called Sleepless.
In 2005, a transketolase-like protein 1 (TKTL1) was identified as a possible mutant form of human TKT [ 4].
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