Methods and Results: We developed 28 primer pairs for L. inflata, all of which amplify CT dinucleotide repeats.
All plates also contained no-template control wells, none of which amplified.
Tectonically derived topographic anisotropy is associated with the three-dimensional kinematic field, which contributes (1) differential surface displacement and (2) crustal weakening along fault structures, both of which amplify processes of surface erosion.
The primers for 5' Sp17 PCR was 5'-GGCAGTTCTTACCAAGAAGAT-3', and that for 3' Sp17 PCR was 5'- GGAGGTAAAACCAGTGTCCTC-3', both of which amplify a complementary DNA (cDNA) of approximately 500 base-pairs.
Design constraints allowed only 26855 primer pairs to be synthesized, some of which amplify the same sequence from 2 genes or gene isoforms.
Hence, Arabidopsis AtGL3 cDNA was exhaustively screened with multiple primer pairs, only one of which amplified a BnGL3 product (~500 bp) (Accession # Pr032754350, www.ncbi.nlm.nih.gov).nih.gov
In this study, we developed 14 polymorphic microsatellite loci for M. lindheimeri, eight of which amplify well in one or more congeneric species.
The primer sequences used were 5′-TGTTTTGTTTTTTTGTGTTTTGGTTTGTGT-3′ (sense) and 5′-CATCCAATCTAAACAACAACAACCACCA-3′ (antisense) for unmethylated DNA and 5′-TGTTTTTTCGCGTTTCGGTTCGCGC-3′ (sense) and 5′-CGTCTAAACGACGACGACCGCCG-3′ antisense) for methylated DNA, both of which amplify ∼15′-CGTCTAAACGACGACGACCGCCG-3′ antisense
Higher expression was confirmed by the use of another couple of primers which amplifies both endogenous and transgenic TIAR mRNAs (Fig. 2B, endo + tg TIAR).
The presence of cDNA in all samples was confirmed by PCR with a set of primers which amplifies a 542 bp segment of the RNF38 ubiquitously expressed gene (data not shown).
These data suggest that rapid activation of p53 results in up-regulation of Klf4, which amplifies the p53-mediated response to IR.
