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For IHC quantitation, original-labeled images were deconvoluted using ImageJ (NIH) to separate hematoxylin (blue; staining all nuclei) and DAB chromagen (brown; sox2-positive nuclei) conditions.
At 40× magnification, nuclei showing expression of Ki-67 antigen in relation to all nuclei were counted.
All nuclei were stained by DAPI.
This, of course, ought to affect all nuclei that decay in this way.
To determine cutoffs for outlier detection, all nuclei were analyzed by principal component analysis.
RNA synthesis recovery was determined by measuring the mean Alexa 594 intensity of all nuclei per well.
At low supercooling all nuclei were of the same chirality as the seed pointing to the seed as their origin.
That the radius is proportional to the cube root of the mass number has the great significance that the average density of all nuclei is nearly constant.
We observed frequent cell division throughout the adult rodent spinal cord, particularly in white matter tracts (5-75-7% all nuclei).
Our results show that the LvGroucho protein localizes to all nuclei throughout embryonic development.
and not all nuclei are likely to decay at the same time so it is impossible to tell when a particular nucleus will decay.
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CEO of Professional Science Editing for Scientists @ prosciediting.com