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All materials were washed with nitric acid and rinsed with nanopure water before use.
To demonstrate the contribution of insoluble substrates rather than soluble extractives released by autoclaving for untreated biomass on hydrogen production, after autoclaving all materials were washed extensively with hot water and the washed residues together with the control without wash were used as the sole carbon source for the cultivation of strain M18.
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All silica materials were washed by small quantities of water and dried at ambient temperature.
All glassware and materials were washed and sterilized in the autoclave at 121 °C for 15 min to prevent any influence by other microorganisms.
In all cases, the resulting materials were washed several times with double-distilled water up to achieving a pH < 10 in the waste liquid and then dried at 60 °C.
The materials were washed with DMF and methanol with a Soxhlet extractor for 2 and 3 days, respectively, prior to the adsorption study.
A team at Plymouth University in the UK spent 12 months analysing what happened when a number of synthetic materials were washed at different temperatures in domestic washing machines, using different combinations of detergents, to quantify the microfibres shed.
Resulting materials were washed with distilled water until pH 7 was reached and dried at 120 °C.
Implant materials were washed with PBS or cell culture medium before use.
The algal materials were washed thoroughly with distilled water to remove debris and other associated biota.
Fixed sporangia materials were washed six times with double distilled water.
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