Sentence examples for all lysates were normalized from inspiring English sources

All lysates were normalized for total protein concentration prior to SDS-PAGE and immunoblotting.

Cell lysates were normalized by protein concentration, reduced with DTT and 10 µg of sample protein were loaded into a 4 12% Bis-Tris gel (Invitrogen).

Lysates were normalized for total protein (10 µg per lane), separated using 4% 20% linear gradient SDS-PAGE (Bio Rad, Hercules, CA), and electroblotted.

The saturation fluorescence values of TG-1 treated cell lysates were normalized for protein content and cell surface thiol contents were normalized for the cell number and averaged using standard SigmaPlot 10.0 software (Systat Software Inc., San Jose, CA).

The cell lysates were normalized for protein content and 500 µg of total protein was incubated with 2 µg of anti-Bax 6A7 monoclonal antibody in 500 µl of Chaps lysis buffer at 4°C for 4 h.

Because the cell lysates were normalized to total cell protein, the dramatic (5-fold) difference in amount of WT and mutant ACE protein (Fig. 3B) might be explained by a reduction in the level of mRNA for mutant ACE (for example due to the effect of this mutation on mRNA stability) or due to intracellular proteolysis of misfolding of mutant ACE.

Lysates were normalized for protein concentration.

Cleared cell lysates were normalized with the BCA assay (Santa Cruz Biotechnology).

Sonicated lysates were normalized to equal amounts of total protein.

Lysates were normalized for protein concentration and were separated on SDS/PAGE (5% gel) by electrophoresis.

Cell lysates were normalized by protein content, and corresponding exosome preparations were adjusted accordingly.