We discuss all key steps of the process, starting from the selection of the appropriate culture medium, quenching techniques to arrest metabolism in a reproducible manner, the extraction of the intracellular components and the profiling of the culture medium.
R. palustris implements all key steps of aromatics degradation including ring reduction, ring cleavage, and β-oxidation on its own [9], [10].
Despite the absence of most genes encoding proteins involved in the production of phytohormones, candidates encoding all key steps of cytokinin production were present in the genome of Pl. halstedii (Additional file 2: Figure S10).
At all the turning points – all the key steps of the Third Reich – he's there.
To date then, there remains no report in the literature on the complete, hands-free automation of all key steps in the processing of ESCs including trypsinization, centrifugation and cell differentiation.
HIF drives transcriptional activation of hundreds of genes involved in vascular reactivity, angiogenesis, arteriogenesis, and the recruitment of endothelial precursor cells, all key steps toward the development of KS [45].
Yet, AF-6 seems to be an unique activator of the parkin/PINK1 pathway since it promotes all key steps, from the translocation of parkin to the mitochondria, through parkin ubiquitin-ligase activation to mitochondrial clustering and mitophagy.
Continuous evolution methods must support all of the key steps of laboratory evolution — translation of genes into gene products, selection or screening, replication of genes encoding the most fit gene products, and mutation of surviving genes — in a self-sustaining manner that requires little or no researcher intervention.
VEGF165 can act through VEGFR-2 to mediate a signaling cascade that regulates all of the key steps of the angiogenic process, including endothelial cell division, proliferation, migration, and tube formation [ 25].
To investigate the role of neddylation in the regulation of angiogenesis, we first determined the effect of neddylation inactivation with MLN4924 using the in vitro rat aortic ring assay that recapitulates all of the key steps of angiogenesis (matrix degradation, migration, proliferation and reorganization).
Concomitantly, 12 transcripts responsible for the key steps of purine, pyrimidine, amino acids and polyamines biosynthesis were all downregulated (Additional file 2: Table S2).
