These mechanisms successfully explain all intermediates observed experimentally.
All intermediates 1, 2, 3, 4, 6, 7, 8 and the final compound 5 were characterized via standard analytical methods.
Minimum-energy structures could be located for all intermediates along putative mechanistic pathways requiring either one (Fig. 4A) or two (Supplementary Figure S9A) zinc ions.
Material details, organic synthesis procedures, analytical characterization of all intermediates including the final Fluselenamyl (1, 2, 3, 4, 5, 6, 7, and 8) and radiochemistry for synthesis of the PET tracer [18F]-9 are described in supporting information.
The block copolymer as well as all intermediates of the synthesis have been fully characterized, for example by nuclear magnetic resonance, gel permeation chromatography and viscometry.
More importantly, if a mixture of all intermediates, cis-PtCl2(NH3)2, trans-PtCl2(NH3)2, and PtCl4 are included simultaneously in the fit, only cis-PtCl2(NH3)2 remains after least-square refinement, the fraction of other components converges to zero.
All these intermediates showed IC50 outranged the standard ellipticine.
Further, all the intermediates have to be selected through evolution.
All intermediate liquid compositions migrate during crystallization to the eutectic.
Note that all intermediate values must be expressible as an integral number of credits.
For the trajectory of each pair of population, all intermediate positions were used to calculate the MOD and MOT.
