Sentence examples for all generated scFv-Fcs from inspiring English sources

All generated scFv-Fcs bound specifically to A. fumigatus and not to other analysed pathogenic Aspergillus species or C. albicans.

Further analysis of the generated scFv-Fc-producing cell clones by qPCR analysis with primers specific for targets other than β-actin might be used for a more in-depth comparison of the generated scFv-Fc-producing subclones.

The finally generated scFv-Fc producer cell lines demonstrate the long-term potency of the target site for RMCE by two different transgene cassettes with varying nucleotide sequence length between the two heterospecific FRT sites.

As expected, flow cytometric analysis revealed strong binding of all three scFv-Fcs to MKN-28 when compared with their binding to HepG2.

All analysed scFv-Fc proteins that recognized conformational epitopes were able to specifically bind to A. fumigatus.

The staining of A. fumigatus for all analysed scFv-Fc clones (Fig. 6A) revealed a pattern on the cell surface on the cell surface, restricted to the growing part of the hyphae.

Utilising the C595 scFv, we generated an anti-MUC1-scFv-Fc-IL2 fusion protein that after binding to MUC1-positive tumour cells induces proliferation of peripheral blood T cells and mediates activation of resting NK cells to lysis of MUC1-positive tumour cells.

In order to optimize plasmids for the transient production in HEK293-6E cells two new test vector variants were generated expressing the antibody scFv-Fc-4E3 or its immunoRNase variant scFv-Fc-RNase-4E3 [11; 4E3 is referred to the CD30 specific scFv], respectively.

Protein A affinity purification and desalting were sufficient to obtain all scFv-Fc antibodies in very high purify of >98%.

Almost all scFv-Fc antibodies were stable for more than half a year storage at 4°C without any protective agent.

During this period of routine cultivation, similar specific growth rates were observed for all scFv-Fc-producing subclones (Fig.  2c).