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To test the efficiency of UAS-lbRNAi constructs we crossed males from all generated lines with Actin-Gal4 virgin females.
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Advertised on buses and in Métro stations, these exhibitions generated lines around the block.
One of the generated lines was able significantly to decrease levels of both Lbe and Lbl proteins Fig. 2G.
We screened ∼700,000 F1 larvae, isolated 591 with fat body GFP expression, and generated lines from each.
Ten percent (9 of 90) of GFP-expressing transgenic fish generated lines with unique GFP expression patterns.
To test whether naturally occurring suppressors were on the second or third chromosome, we generated lines with mixed autosomal backgrounds.
We first generated lines that were Col/Ct heterozygous within 420 and Col/Col homozygous outside (HET-HOM).
By passage 6, the generated lines were tested for the ES-specific antigens SSEA4, TRA-1-60, Oct4 and Nanog.
All of these mice were compared with a previously generated line of mice that harbor the same PrP-N171-82Q construsedused in the co-injection studies described here.
The purple quadrangles represent control points and the red line represents generated line Fig. 7 Point cloud classification.
If any of the measures does not satisfy the minimum constraints, the rule is not generated (line 29).
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