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All generated constructs were verified by sequencing.
The pUC18 plasmid was utilised as the backbone for all generated constructs.
The sequence of all generated constructs was verified and confirmed by restriction enzyme mapping and DNA sequencing.
All generated constructs were verified in both directions by Sanger sequencing and using the human sequence GRCh37.p13 as a reference.
All generated constructs were introduced into Arabidopsis thaliana ecotype Columbia (Col-0) by Agrobacterium tumefaciens mediated transformation via the described floral dip method [ 43].
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Sequences of all PCR generated constructs were verified by DNA sequencing.
All PCR generated constructs were verified by sequencing.
All deletion boundaries or PCR generated constructs were verified by sequencing.
QHZ generated constructs over-expressing miR172a and miR172b, analyzed transformed plants and performed all molecular analyses.
The set of generated constructs shows which aspects of a particular topic play a role in its perception.
We're no closer to getting at the heart of art, deciding whether these generated constructs count as art, and if so, by whom, but it's interesting nevertheless.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com