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All experimental steps were performed in duplicate.
Since it is possible to control all experimental steps by adequate software, it is then possible to make real time opto-digital holographic microscopy.
Indeed, a statistical survey of our entire population shows that the baseline firing rate remained constant through all experimental steps.
We therefore limit the use of a 5 kGy γ-irradiation to the decontamination of water used at all experimental steps.
All experimental steps were carried on ice.
LAV was involved in all experimental steps and drafting of the manuscript.
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INAA is well known as reference analytical technique because all the experimental steps are totally traceable and there is absence of physical-chemical sample manipulation reducing the positive and/or negative artifacts formation [7, 9, 20, 21].
Single cell reduced representation bisulfite sequencing (scRRBS) integrates all the experimental steps before PCR amplification into a single-tube reaction to avoid unnecessary DNA loss and enables the detection of approximately 40% of the CpG sites in comparison with standard RRBS using thousands of cells [68, 69].
All the experimental steps (chemical characterization, dilute acid pretreatment, enzymatic hydrolysis, and alcoholic fermentation) were carried out in triplicate, and the results were calculated as means ± standard deviations.
Created tool presented in this paper allows fast, pre-synthesis screening of ILs, with the omission of any experimental steps.
All the further experimental steps including the transformation of E.coli DH5alpha were done according to the instructions of the Gateway Clonase LR II enzyme kit (Invitrogen).
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