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ESCs were grown under feeder-free conditions and used for all ESC experiments.
We also examined methylation status at the REX1 locus in previously published human ESCs and iPSCs from Ziller et al. (2011), and found that although all ESC lines examined exhibited consistent levels of low methylation at the REX1 promoter, human iPSC lines analysed in exhibited either hemi- or hyper-methylated REX1 promoter regions.
Second, although ESCs can contribute to chimeras and support full development, not all ESC cultures are able to do so.
All ESC and GCT mRNA array data were implemented using the Affymetrix Human Genome U133 Plus 2.0 chips.
ERCC5 is a gene enriched in all ESC, HSC, and NSC, like ABCB1, and is involved in DNA repair.
All ESC and iPSC lines infected with EOS were cultured for 7 days in a medium supplemented with puromycin.
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All these ESC lines are indistinguishable from wild-type ESCs in culture.
Table 2 lists all the ESC lines used and their associated genotype.
The derivation efficiency could be further increased by expanding all early ESC clones instead of selecting them on the basis of their morphology and growth rate (Table 1).
The results showed that ESC, ESC-2 all had inhibitory effects on 4 tumor cells.
All ESC-RCC were positive for CK20 and melanocytic markers Melan-A, Cathepsin-K or HMB45, as well as PAX8, whereas EMA, pan-cytokeratin, CK7, CKIT, CD10, CAIX were negative.
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