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All determinations were done weekly between 0800 and 0900 hours.
All determinations were done at least in triplicate [see Additional file 2].
All determinations were done at least in duplicates.
All determinations were done in triplicate.
All determinations were done by triplicate.
All determinations were done within one single assay.
Similar(45)
Samples were immediately frozen at −20°C then stored at −80°C, and all the determinations were done at the same time (2 years after the first inclusion).
Total N determinations were done by the Kjeldahl method of Bremner [29].
Urea determinations were done following the method of Goeyens et al. (1998).
Total N determinations were done by the Kjeldahl method of Bremner (1965).
Five separate experimental determinations were done for the natural frequency of each specimen and then the average value was adopted.
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