Taken together these results indicate that IntI1 possesses all the determinants required for the attC recombination reaction and that the bsattC intermediate can be generated during the reaction time in absence of other protein in addition to the recombinase.
A library of alanine substitutions in α was used to identify the αCTD determinants required for Fis-dependent transcription at rrnB P1 and rrnE P1.
Identifying the structural determinants required for receptor binding of Bv8 PKs is mandatory for the design of PKR antagonists, which may be useful in the treatment and prevention of various disease states.
The full-length hepatitis C virus (HCV) JFH1 genome (genotype 2a) produces moderate titers of infectious particles in cell culture but the optimal determinants required for virion production are unclear.
To initiate the analysis of structural determinants required for FTCD function in vimentin dynamics, we used structure-based design to generate individual formiminotransferase (FT) and cyclodeaminase (CD) domains, and to produce an enzymatically inactive FTCD.
Functional determinants required for allosteric control identify: (i) an important link between the ATP bound ribose moiety and the SensorII motif that would allow nucleotide-dependent α-helical α/β subdomain dynamics; and (ii) establishes a novel regulatory role for the SensorII helix in PspF, which may apply to other AAA+ proteins.
These data suggest that the determinants required for CFH binding are defined by conformation rather than contiguous linear elements.
Most of the variants did interact with hCG1, UNG2 and DCAF1, as visualized by growth of yeast-transformed cells on medium without histidine (Fig. 3), arguing for a good conservation of the structural determinants required for Vpr functions.
We could note that the two cysteine residues, that are critical for binding to p56Lck ([27], and data not shown), are not required for binding to PLSCR1 (right panel, mutant CD4 C/S), indicating that the determinants required for interaction with PLSCR1 are different from those involved in the interaction with p56Lck.
To shed more light on virulence determinants required for evolution of HPAIV which, in nature, derive from low-pathogenic precursors of subtypes H5 or H7 [9], [10] [11], [12], [13], [14], [15], [16], [16], we addressed the question whether a low-pathogenic avian strain of the H5N1 subtype would transform into a highly pathogenic strain after introduction of a polybasic HA cleavage site.
