QC and initial data preparation were performed using the software package plink [ 29].
Preliminary miRNA microarray data preparation was performed using the Affymetrix miRNA QCTool [ 50].
Cytogenetic harvests and slide preparations were performed using standard protocols.
Library preparation was performed using NEBNext® Ultra™ DNA Library Prep Kit for Illumina® (New England Biolabs, Inc. USA).
Adenovirus preparation was performed using Vivapure AdenoPACK Kit (Sartrius) according to the manufacturer's instructions.
Library preparation was performed using a SQK-LSK108 Sequencing Kit R9.4 version (Oxford Nanopore Technologies, Oxford, UK) using 500 ng of DNA.
Library preparation was performed using the Nextera XT library preparation kit (Illumina Inc., San Diego, CA, USA), and 2 × 300 bp paired-end sequencing performed on the Illumina MiSeq platform using a V3 sequencing cartridge, to ~50 × coverage.
Library preparation was performed using 150 ng high-quality RNA (TruSeq Library Prep Kit, Illumina), and sequenced on a HiSeq 2000 (Illumina).
Femoral canal preparation was performed using rasps alone.
The nano-probe preparation is performed using the STM-TEM holder within a 200-kV HRTEM.
