After long-term culture in SCM, however, NSE was significantly upregulated in all the GBM cell cultures except for A172, in which NSE was downregulated (0.4 ± 0.12, p = 0.002, figure 2A).
All experiments were performed on confluent cultures except for those experiments using antibody visualization, subconfluent cells of approximately 70% confluence were used for optimal antibody coverage.
All experiments were performed in triplicate, using different cell cultures, except for the comparison of mid-log versus stationary cultures, which was done only once.
Conversely, expression of Bax and Bid in 𝒟lentiginosine-treated cells was significantly or highly significantly increased with respect to corresponding control cultures in all cell lines assayed after 3 h as well after 18 h in cultures, except for HT-29 cells after 3 h of treatment.
In general, enzymes involved in butyrate synthesis were detected in higher amounts for both growth phases in high glycerol cultures, except for acyl-CoA dehydrogenase (CBY_3258), which showed lower expression levels in both exponential and stationary growth phases in high initial glycerol concentration cultures.
As followed from the Table 3, surface charges of exponentially growing bacteria did not differ significantly from those of stationary phase cultures, except for A. simplex (zeta potential increased from −38.8 to −24.6 mV), B. subtilis (zeta potential increased from −37.8 to −31.3 mV) and P. fluorescence (zeta potential decreased from −20.5 to −26.4 mV).
The proportional distribution of PCR-detected serotypes from culture negative cases was similar to the serotype distribution of isolates identified from CSF cultures, except for serotypes 1 and 24, which ranked 2nd and 23rd, respectively, among the cultured isolates, compared to 6th and 3rd, respectively, among the serotypes detected from culture negative cases by PCR (Figure 4A, 4B).
Data presented below are the representative results from the three prolonged spheroid cultures, except for where specified.
Elicitors were added to 3 weeks old hairy root cultures except for the treatments with elevated sucrose concentrations which occurred for the whole culture period.
Other cultures were supplied as pure cultures except for a few cases where those organisms depended on smaller protozoa as a food source.
