Exact(3)
All colorimetric measurements were performed at room temperature in the detection solution (25 mm HEPES, 20 mm KCl, 200 mm NaCl, 0.05% Triton X-100, 1% DMSO, pH 8.0) by using UV−Vis absorption spectroscopy.
For all colorimetric assays, purified enzymes were diluted in 5 mM MES buffer pH 6.5 to a concentration of 40 ng μL 1.
A HistoQuest marker profile was created to save all colorimetric and thresholding parameters and was used in all analysis projects of the study.
Similar(57)
Regression analysis of all DPD colorimetric analyses with the HPLC values produced a linear model (Y=0.9602 X+0.1259) with an r2 value of 0.9960.
As shown in Figure 1, the soybean lipoxygenase activity on linoleic acid was detectable by all three colorimetric methods.
Accumulation of glutamate in the culture medium was assayed by monitoring the reaction catalysed by glutamate dehydrogenase, using iodonitrotetrazolium chloride as a colorimetric indicator, all supplied as a kit (R-Biopharm).
Total antioxidant status (TAS) was assessed in all serum samples by a colorimetric assay based on the decrease of the optical density of the blank produced by each sample in analogy to its antioxidant property (Randox Laboratories, San Francisco, CA, USA).
Arsenite collected from the flow through can be detected using a colorimetric assay in which all arsenic species are first reduced into arsine gas within an enclosed compartment.
To confirm the presence of Aβ in the choroidal vasculature, another method of immunohistochemistry was performed using a colorimetric staining to rule out all background staining that might result in false positive staining.
Urinary creatinine was also measured for all participants in the same laboratory by a colorimetric method based on the Jaffe reaction (Heinegård and Tiderström 1973).
For all coatings, the colorimetric coordinate variation is definitely lower than the intrinsic unevenness of untreated bronze specimens.
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