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Exact(6)
All blots were normalized with β-tubulin.
All blots were normalized with an antibody against β-actin (42 kDa, Jackson ImmunoResearch Laboratories) and protein was visualized with an enhanced chemiluminescence (ECL) labeling kit (Santa Cruz Biotechnology) according to the manufacturer's instructions.
Protein band intensities for each human colon cancer sample, in all blots, were normalized to the mean protein band intensities from duplicate HCT116 total protein sample present in the same blot, and similarly included in all blots.
All blots were normalized to GAPDH (#AM4300; Ambion, Austin, TX, USA) or to actin (#sc-1616; Santa Cruz).
β-actin was used as a loading control and used as an internal control, and all blots were normalized to β-actin.
All blots were normalized to Tubulin (1 : 5000, Santa Cruz Biotechnology, Santa Cruz, CA, USA) and at least three independent experiments were performed.
Similar(54)
The same blots were normalized against anti-ERK1/2 or anti-Akt antibodies (1∶1000; New England Biolabs).
Western blots were normalized with GAPDH.
Western blots were normalized using the anti-β-actin antibody.
Blots were normalized by probing with an antibody against NSE.
Blots were normalized to total p38 and ERK½, respectively (representative blots shown).
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