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"He said: 'You are an artist, because you have that combination of remaining vulnerable to what's around you and yet have a backbone of steel so you don't give a bugger what anybody says about it.'" You would think she was all backbone, all steel.
The N-methylation reaction considers all backbone amide nitrogens derived from proteinogenic and nonproteinogenic amino acids as potential sites of N-methylation.
The most flexible interactions are Glu-Lys salt bridges, which can act like tethers to bind strands even after all backbone interactions between the strands have been broken.
After this a further combination of 5000 steps of steepest descents and 5000 steps of conjugate gradients energy minimisation were carried out in which the same restraining force was applied to all backbone atoms only.
The incorporation of xCSA with multi-dimensional 13C/15N correlation is demonstrated with a GB1 protein sample as a model system for measuring 13C/15N CSA of all backbone 15NH, 13CA and 13CO sites.
Open image in new window Fig. 2 Plots of the running average of the RMSD calculated for all backbone atoms during MD simulations with and without capping (nc) using the ff99SB/TIP3P and ff02.r1/POL3 force field and water potential combinations: a c-Fos c-Jun c-Fos c-Junc-Fos c-Jun complex.
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Modelling accuracy was tested using the all-backbone-atom (C-α, N, C, O) RMSD between a model and the 'native' target X-ray structure, as found in the PDB.
All the backbone of an Irukandji these Aussie batsmen and not nearly as deadly".
Thirty-something, married with two daughters, sexually confident but emotionally bewildered, Ben has all the backbone of a chocolate éclair.
But first they had to build it, a task that required all the backbone Ms. Lambert revealed in her initial letter to her father.
B RDEE only requires that the three most expensive steps of the design algorithm be performed once, simultaneously for all backbones (Section 1.3, paragraph 3).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com