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All assay points were determined in triplicate and all experiments were repeated three times.
L, progeny viability of PE matings was greater than that for DE matings (Fig. 3a) for some but not all assay points.
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Although relatively modest, the lifespan extensions by unc-31 and eat-18 mutations were reproducible and sometimes significant; they thus provide informative assay points that contribute to all measures of correlation, and in particular to rank-order correlations.
Twelve standard assay points were used to generate a standard curve.
The GTP hydrolysis assay points towards an ARHGEF7-mediated, significant twofold induction of LRRK2 GTPase activity.
The GTP hydrolysis assay points to a twofold increase of LRRK2 GTPase activity in the presence of ARHGEF7.
Two sites, each located 150 kb outside of the centromere, one in Xp (ZXDA) and one in Xq (site 9), were non-centromeric assay points.
Higher nanofractionation frequencies yield more assay points per separated peak, thereby providing better resolution capabilities.
However, this pattern was reversed as evolution progressed: the last two assay points showed significant differences (PE > DE, Fig. 3).
The ANOVA included only assay points 6 and 7, the most derived states of the lineages (Table 1).
Assay points 1 7 were designated at the end of each of the seven sexual-asexual propagation cycles.
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