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All assay procedures were performed at room temperature.
All assay kits were purchased from Promega, Southampton, UK.
Centaurea cheirolopha extract, with the highest amount of total phenolic and flavonoids, showed the highest antioxidant activities in all assay, except for metal chelating.
However, standardized and comprehensive methods for all assay steps involved need to be developed before detection of micrometastasis are incorporated into routine clinical practice.
An analyst visually reviewed all assay results prior to data upload.
All assay points were determined in triplicate and all experiments were repeated three times.
All assay validation data were generated by using dilutions of plasmids containing the respective assay target sequences.
The template DNA was diluted in series, combined with all assay probes (35 BCOs and 20 RCOs) and subjected to ligase discrimination.
Notably, Lfng protein was easily detected in CD4+ T cells under all assay conditions as well as naïve CD4+ T cells from different genetic backgrounds (data not shown).
Express across all assay sites.
All assay results were negative.
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CEO of Professional Science Editing for Scientists @ prosciediting.com