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All additional samples (A1-A10), as well as sample AC (control-no purification method), were sequenced.
Monthly sample collection was conducted by trained field technicians, while farm personnel were trained to collect all additional samples using aseptic methods [ 18].
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All the additional samples, 2073 young-onset cases and 2045 UK population controls were tested for the R798X sequence variant by the 5′nuclease assay (Taqman™) using the ABIPrism 7900HT sequence detection system according to the manufacturer's instructions.
If any additional samples are required during the visit, expect to pay about £20 plus VAT per sample.
Ask if any additional samples are available.
Again, across all 3 additional samples, the medial temporal cortex showed the largest magnitude of thinning of all regions within each sample, ranging from 0.26 to 0.55 mm thinner in AD than OC.
To increase the sensitivity of the PCR specific primers targeting GBoV1 sequence were used to screen all samples, but no additional samples were found positive.
The results presented here are based on a re-analysis of our original GWAS dataset, including additional samples, all called using the Illuminus program (36).
The independent variant identified at the LPP locus in our American Indian GWAS for type 2 diabetes was replicated in the additional samples (all American Indian meta-analysis, p = 8.9 × 10−6, OR 1.29 [95% CI 1.15, 1.45]).
Additional samples from all major organs were fixed in 10% buffered formalin.
Additional samples from all joints were fixed in 10% neutral buffered formalin and embedded in paraffin wax, sectioned (4 μm) and stained with haematoxylin and eosin (H&E).
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