We found that PGCs in both male and female GR-absent embryos still expressed PGC characteristic markers, including Stella, SSEA-1, Blimp1, Dazl and the pluripotent marker Oct4.
Changes were absent in embryos exposed at later stages, i.e., 12 hpf.
So, we analyzed the expression of krox20, a marker for dorsally derived hindbrain segments rhombomere 3 and 5. Krox20 expression was completely absent in embryos treated with 12.5 µM of DTAB at 4hpf onwards (Fig. 3 A B).
Our detailed analyses indicated that several important pathways active in mature plant tissues are almost absent in embryos.
Our expression studies show that Syp is absent from embryos but is highly expressed in the larval nervous system.
However, at E12.5, phosphorylation of Akt in p110αD933A/D933A mutants is absent and embryos are lethal, exhibiting primary angiogenic remodeling defects (Graupera et al., 2008).
MHCα was undetectable at earlier stages but by stage-27/28 stage-27/28 when increasing, MHCα expression was absent in embryos injected with Xic1Mo, through to stage-33/34.
LIN-41 is present in the oogenic germ line from the mid-pachytene stage, but begins to be eliminated during meiotic maturation and is absent from embryos.
These findings can now be explained by our demonstration that in the presence of a disrupted or absent ZP, embryos lose membrane integrity when exposed to proteases similar to those present in the oviduct during the post-ovulatory time period.
Except for those embryo sacs that degenerated, structural abnormalities were absent from embryo sacs of AtESP-RNAi plantsuggestinging that reduction in AtESP levels slowed or delayed the developmental program, but did not block it.
